Vitamin D Test - Bloodspot

Vitamin D Test - Bloodspot

25-Hydroxyvitamin D is the major circulating form of vitamin D, occurs in 2 forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol), and is the precursor of the active form (1,25-dihydroxyvitamin D). Because of its long half-life, measurement of total 25-Hydroxyvitamin D (D2 plus D3) provides the best assessment of vitamin D status and includes vitamin D derived from diet, supplements and exposure to UVB light (e.g. sunlight). ThisLC/MS QQQ method is sensitive and specific for both Vitamin D2 and D3, and each form is measured and reported independently.


Vitamin D is best known for its role in calcium and bone metabolism but emerging research indicates that low levels of vitamin D may be associated with increased risk of some cancers, type 2 diabetes mellitus, multiple sclerosis, cardiovascular disease, rheumatoid arthritis, depression, Alzheimer’s disease, infections, preeclampsia, cesarean deliveries and neurocognitive dysfunction. Vitamin D regulates the expression of a vast arrayof genes in tissues including immune cells, the vasculature, muscle and reproductive organs. Vitamin D insufficiency is common and deficiency can have adverse health effects at any stage of life. Many testing methods do not differentiate between the 2 forms of Vitamin D and only total concentrations are reported.


Reference Intervals


Due to geographic location, ethnic background, and seasonal variation, population-based reference values for vitamin D do not correlate well with clinically relevant vitamin D effects and are of limited clinical value. The following reference intervals are similar to those of the 2011 Endocrine Society Practice Guidelines and apply to males and females of all ages. The optimal level for Vitamin D is above 55, but below 100.


  • < 10 ng/ml (< 25 nmol/L) - severe deficiency. May be associated with osteomalacia or rickets (children). Serum calcium andphosphate may be low and, parathyroid hormone and serum alkaline phosphatase may be abnormally high.
  • < 20 ng/ml (< 50 nmol/L) - deficiency. Increased risk of osteoporosis and secondary hyperparathyroidism.
  • 20 - < 40 ng/ml (50 - < 100 nmol/L) - moderate deficiency to suboptimal. In addition to insufficient intake and exposure to UVB light, consider malabsorption syndromes (e.g. pancreatic insufficiency, Celiac or Crohn’s disease), hepatic or kidneydisease, and prolonged use of medications such as antifungals, antiseizure drugs, cholestyramine and glucocorticoids.
  • 40 - 80 ng/ml (100 - 200 nmol/L) - optimal levels in a healthy population.
  • > 100 ng/ml (> 250 nmol/L) - elevated.
  • Toxicity is usually associated with vitamin D levels > 150 ng/ml (> 375 nmol/L) for prolonged periods of time.



  1. Peterlik M, Cross HS. Vitamin D and calciuminsufficiency-related chronic diseases: anemerging world-wide public health problem. Int J Environ Res Public Health. 2009;6:2585-2607.
  2. Holick MF, Binkley NC Bischoff-Ferrare HA, et al. Evaluation,Treatment, and Prevention of Vitamin D Deficiency: An Endocrine Society Clinical Practice Guideline. J ClinEndocrinol Metab (2011)96(7):1911-30.
  3. Institute of Medicine (US)Committee to Review Dietary Reference Intakes for Calcium and Vitamin D, Washington, DC: The National Academies Press, 2011.
  4. Souberielle JC, Body JJ, Lappe JM et al. Vitamin D and Musculoskeletal Health, Cardiovascular Disease, Autoimmunity and Cancer: Recommendations for Clinical Practice. Autoimmune Rev (2010)9(11)709-15.
  5. Chen P, Hu P, Xie D, et al. Meta-analysis of Vitamin D, Calcium, and the Prevention of Breast Cancer. Breast Cancer Res Treat (2010)121(2):469-77.
  • Collection Instructions and Testing Information

    1. Please review the test collection instructions. Click Here
    2. For more information regarding the Vitamin D test please visit the Doctor's Data website.
    3. Example of a Sample Report.
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